CCM-300 葉綠素含量測量儀
- 公司名稱 北京澳作生態儀器有限公司
- 品牌
- 型號 CCM-300
- 產地
- 廠商性質 生產廠家
- 更新時間 2024/10/15 8:38:48
- 訪問次數 1157
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產地類別 | 進口 | 價格區間 | 面議 |
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應用領域 | 環保,農業 |
葉綠素含量測量儀廣泛應用于植物生理生態研究,特別是對傳統吸收法測量困難的小葉片或樣品的葉綠素含量進行測量,例如針葉、發育未*的水稻、生于巖石上的絲狀藻、地衣、草坪草、仙人掌、龍舌蘭屬植物、菠蘿、擬南芥、果實、苔蘚、葉莖、葉柄。
二原理
盡管實踐已經證明了吸收技術測量葉綠素含量的有效性,但該方法對樣品的要求較高,使其應用范圍受到限制。而樣品本身的葉綠素熒光則不受樣品厚度、均一性等的影響,可以指示葉綠素的含量。根據這個原理,Gitelson等比較了葉片的吸收和反射對葉綠素熒光的影響,發現在735nm和700nm處的比值與葉片的反射相關性最高,并且當樣品葉綠素含量在41mg·m-2到675mg·m-2時,F735/F700的比值與其葉綠素含量間存在著顯著的線性關系(R2≥0.95),與傳統的吸收性測量方法相比(測量范圍≤300mg·m-2),提高了葉綠素含量的線性關系范圍。
根據Gitelson的研究結果,CCM-300使用具有15nm半峰寬的460nm二極管用于熒光激發,提高了紅色熒光波長,避開了大約685nm的發射熒光峰(葉綠素可重新吸收,降低測量精度)。儀器使用在735nm和700nm的葉綠素發射熒光的比率(F735/F700)進行葉綠素含量的測量,讀數為F735/F700的比值以及根據Gitelson方程計算的葉綠素含量。
三 特點
Ø 直接算出葉綠素含量mg/m2;
Ø 測量結果可靠,不受葉片或樣品大小、厚度和形狀的影響;
Ø 經濟、輕便、手持式設計,適合野外使用;
Ø 與化學測量結果高度相關,即使在高葉綠素含量時;
Ø 彩色觸摸屏顯示;
Ø 離散的單點測量和多點(2-30個樣品)平均值測量模式;
Ø 非易失性閃存(2GB);
USB數據輸出,可使用Excel打開; |
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四 組成
主機、光纖樣品夾、電池充電器、4節AA鎳氫充電電池、USB通訊纜線、使用手冊。
可選配件:
20mm大探頭: 增加測量面積。 較大的樣本量可能對某些類型的樣本有用,例如苔蘚
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增長版葉夾:
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Ø 測量參數:葉綠素熒光比率——發射熒光在735nm/700nm的比值(CFR),或相對葉綠素含量mg· m-2;
Ø 測量面積:直徑3mm的圓,外徑4mm,但對直徑小于3mm的樣品也可準確可靠的測量;
Ø 分辨率:比值0.01或1 mg· m-2;
Ø 重復性:依賴于信號強度,對信號強度低的樣品,建議使用多點測量的平均值,對信號強度高的樣品,差異通常為±0.03或更低;。
Ø 噪聲:<±0.02;
Ø 光源:LED 460 nm藍光二極管,半峰寬15 nm;
Ø 探頭:兩個固態高感傳感器,提供濾光片。同時提供兩個波長探頭(700nm~710nm,730nm~740nm);
Ø 檢測:數控調制光,將背景光影響將至低。并對光源和檢測器進行溫度補償;
Ø 存儲容量:2GB;
Ø 模式:單點測量,2~30個點去平均值,去除2σ區間外的平均值,或者中位數;
Ø 樣品接口:光纖傳感器,直徑4mm;
Ø 用戶界面:240 × 320 像素彩色觸摸屏;
Ø 輸出:USB 1.1;
Ø 溫度范圍:0~50 ℃;
Ø 電源:2節充電AA電池;
Ø 自動關閉間隔:(無按鍵或下載)0~20分鐘可選;
Ø 大小:12 cm × 9 cm × 3 cm;
Ø 重量:0.16lbs 275g;
Ø 測量時間:5秒;
五、產地:美國
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