3D單分子熒光成像系統(tǒng)-SAFe 360簡(jiǎn)介：

SAFe 360是法國(guó)abbelight公司推出的款基于單分子定位的顯微成像（SMLM）的新型3D單分子成像系統(tǒng)，它*的DAISY技術(shù)整合了散光技術(shù)和超臨界角光技術(shù)，能夠很大的提高定位精度，xyz三軸定位精度高達(dá)15nm，可以提供高清晰三維亞細(xì)胞結(jié)構(gòu)圖像，支持同時(shí)多四色成像，可以用于細(xì)胞納米三維成像，觀測(cè)高清晰亞細(xì)胞器結(jié)構(gòu)，實(shí)時(shí)研究不同的結(jié)構(gòu)功能蛋白的共定位信息，在單分子水平研究分子動(dòng)力學(xué)反應(yīng)以及細(xì)胞間的相互作用等。

3D單分子熒光成像系統(tǒng)-SAFe 360設(shè)備參數(shù)

+ 成像模式：PALM、STORM、PAINT、smFRET 、SPT

+ 光源模式：Epi、TIRF、HILO

+ 分辨率：15 nm的XYZ軸分辨率

+ 超大視野：200 × 200 μm2的視野

+  次可同時(shí)采集1.2 μm深度圖像信息

+  圖像深度：10 μm

+  實(shí)時(shí)漂移矯正

+  四色同時(shí)成像

+  活細(xì)胞成像模式

配套試劑

測(cè)試數(shù)據(jù)

發(fā)表文章

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[12] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.

[13] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.

[14] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.

[15] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177.

[16] Bouissou, Ana?s, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040.

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