Dionex DNAPac PA100 寡核苷酸色譜柱
- 公司名稱 北京豫維科技有限公司
- 品牌 DIONEX/英國
- 型號
- 產地
- 廠商性質 代理商
- 更新時間 2019/11/12 15:21:59
- 訪問次數 1739
聯系方式：水小松18911849923 查看聯系方式
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
經營理念：：“誠實守信、專業技術、優質服務”
北京豫維科技有限公司是一家服務于色譜、光譜、質譜、實驗室等技術服務的供應商，專門為國內的化學分析用戶提供專業的實驗室設計、儀器配備、耗材供應、分析方法開發和技術咨詢等服務。 公司有著多年的經驗積累，我們以優質的服務為您提供幫助！ 我們秉承：“誠實守信、專業技術、優質服務”。我司主要經營品牌：安捷倫Agilent、島津Shimadzu/GL Sciences 、鉑金埃爾默PE、熱電-賽默飛ThermoFisher 、戴安Dionex、沃特世Waters 、瑞思泰康Restek 、杰斯特 GS-Tek、 菲羅門Phenomenex、德國MN、 美國VICI 、澳大利亞SGE、沃森wasson-ece、光譜Spectra/Linde 、液化空氣Scott/Air Liquide、Entech 采樣罐等品牌。
蘇瑪罐，原子吸收分光光度計，環境監測產品，液相色譜，色譜柱。
| 供貨周期 | 現貨 | 規格 | 4 x 250 mm |
|---|---|---|---|
| 貨號 | 043010 | 應用領域 | 醫療衛生,環保,食品,化工,石油 |
Dionex DNAPac PA100 寡核苷酸色譜柱
Thermo Scientific? Dionex? DNAPac? PA100 寡核苷酸柱的特點是采用強陰離子交換樹脂，從而可對合成型和經過修飾的寡核苷酸進行高分辨率分析和純化。
描述
13μm、薄膜型、無孔、聚合物樹脂，并帶有與季胺官能團鍵合的 MicroBeads?
可對寡核苷酸進行 n、n-1 分離
解析次級結構的寡核苷酸
與溶劑、高 pH 和高溫相容
分析基于硫代磷酸酯的臨床樣品
可輕松升級
Dionex DNAPac PA100 寡核苷酸色譜柱
訂貨信息：
| 分析柱 | |
| DNAPac PA-100 Analytical Column (4 x 250 mm) | 043010 |
| DNAPac PA-100 Semi-Preparative Column (9 x 250 mm) | 043011 |
| DNAPac PA-100 Semi-Preparative Column (22 x 250 mm) | SP2091 |
| FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 150 | SP4008 |
| FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 600 | SP3229 |
| DNA Pac PA-100 Analytical Column (2 x 250 mm) | SP3686 |
| DNAPac PA-100 Column (22 x 50 mm) | SP4513 |
| FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 10 | SP4757 |
| 保護柱 | |
| DNAPac PA-100 Guard Column (2 x 50 mm) | SP4016 |
| DNAPac PA-100 Guard Column (4 x 50 mm) | 043018 |
For n, n-1 Resolution of Single-Stranded Oligonucleotides
The DNAPac? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.
Capable of n, n-1 resolution for oligonucleotides
Resolves oligonucleotides with secondary structures
Compatible with solvent, high pH, and high temperatures
Analyzes phosphorothioate-based clinical samples
Provides easy scale-up
The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.
Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.
Separates RNA / Double-Stranded DNA
The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.
Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.
| DNAPac? PA100 for n, n - 1 Resolution of Single-Stranded Oligonucleotides Specifications | |
|---|---|
| Temperature range | 4–90 °C |
| Typical operating conditions | 2400 psi at 1.0 mL/min (analytical and guard columns) Recommended flow rate: 1.0 mL/min |
| Mobile phase compatibility | pH 2–12.5; 100% compatible with common organic solvents. Ionic form eluents: chloride, acetate, or bromide in lithium, sodium, or ammonium forms. |
| Resin composition | 13 μm-diameter nonporous substrate (55% cross-linking) agglomerated with a 100 nm MicroBead alkyl quaternary ammonium functionalized latex (5% cross-linked). |
| Anion-exchange capacity | ~40 μeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK? with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Dimensions | DNAPac PA100 Analytical Column: 2 × 250 mm DNAPac PA100 Guard Column: 2 × 50 mm DNAPac PA100 Analytical Column: 4 × 250 mm DNAPac PA100 Guard Column: 4 × 50 mm DNAPac PA100 Semipreparative Column: 9 × 250 mm DNAPac PA100 Semipreparative Column: 22 × 250 mm DNAPac PA100 Semipreparative Guard Column: 22 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
采購中心
化工儀器網