Organ-on-a-Chip片上器官組織內(nèi)皮屏障

說(shuō)明

這種芯片的設(shè)計(jì)可以模擬1）三維組織和內(nèi)皮屏障的形成，2）它們的交互作用。該裝置包括狹縫或間隙，以形成外部通道和內(nèi)部腔室之間的交叉點(diǎn)。通過(guò)這種方式，有可能重建對(duì)可靠組織模型（如血腦屏障和其他內(nèi)皮/組織界面）至關(guān)重要的緊密和間隙連接。

重建三維組織模型的可能性通過(guò)提供更準(zhǔn)確地描述體內(nèi)真實(shí)情況的生物和形態(tài)學(xué)微環(huán)境以及確保方便的實(shí)時(shí)可視化，加速了細(xì)胞行為和藥物篩選的實(shí)時(shí)研究。

通道尺寸、組織腔尺寸、支架和屏障設(shè)計(jì)有多種選擇（見(jiàn)規(guī)范）。我們可以支持您的選擇，并根據(jù)您的需要選擇正確的參數(shù)。

這種智能和創(chuàng)新的設(shè)計(jì)克服了當(dāng)前流動(dòng)室或基于跨井室的分析固有的局限性。目前的流動(dòng)腔設(shè)計(jì)過(guò)于簡(jiǎn)單化，描繪了微環(huán)境的尺度和幾何結(jié)構(gòu)，無(wú)法對(duì)輪回進(jìn)行建模。同樣，在活體內(nèi)觀察到的流體剪切和尺寸/拓?fù)浣Y(jié)構(gòu)也不在井腔中，遷移的終點(diǎn)測(cè)量不可重復(fù)，也不能提供實(shí)時(shí)可視化。

這種簡(jiǎn)化和理想化的微血管網(wǎng)絡(luò)很容易再現(xiàn)細(xì)胞分層、恒定剪切和流動(dòng)條件。

好處：

并行架構(gòu)實(shí)現(xiàn)了定量實(shí)時(shí)可視化

具有工程多孔結(jié)構(gòu)的生理性滲漏血管

物理上真實(shí)的對(duì)流和擴(kuò)散輸運(yùn)

超低消耗量微氟平臺(tái)

高抗光透明材料

默認(rèn)顯微鏡玻片尺寸

選項(xiàng)（請(qǐng)參閱“規(guī)格”選項(xiàng)卡中的可用設(shè)計(jì)和選項(xiàng)詳細(xì)信息）：

設(shè)計(jì)1

方案A

1）100m狹縫間距-2m寬狹縫-50m屏障寬度

2）50 m狹縫間距-3 m寬狹縫-50 m屏障寬度

3）50 m狹縫間距-3 m寬狹縫-100 m屏障寬度

4）50 m狹縫間距-2 m寬狹縫-50 m屏障寬度

5）50 m狹縫間距-2 m寬狹縫-100 m屏障寬度

方案B

1）3米柱直徑-3米間隙-100米屏障寬度

2）3 m柱直徑-8 m間隙-50 m屏障寬度

3）3 m支柱直徑-3 m間隙-50 m屏障寬度

4）3 m柱直徑-8 m間隙-100 m屏障寬度

5）8m支柱直徑-3m間隙-100m屏障寬度

6）8 m m柱直徑-8 mm間隙-50 mm屏障寬度

設(shè)計(jì)2

方案A

1）50 m狹縫間距-2 m寬狹縫-100 m屏障寬度

方案B

1）3m支柱間隙

2）8m礦柱間隙

Organ-on-a-Chip片上器官組織內(nèi)皮屏障

Description

The design of this chip allows to mimic 1) the formation of 3D tissues and endothelial barrier and 2) their cross-talk interactions. The device includes slits or gaps to form the intersection between the outer channel and inner chamber. In this way it is possible to recreate tight and gap junctions essential for a reliable tissue modelling (such as the blood-brain barrier and other endothelial/tissue interfaces). 

The possibility to recreate 3D tissue models accelerates real-time studies of cellular behavior and drug screening by providing a biological and morphological microenvironment that more accurately depicts in vivo reality and ensuring a convenient real-time visualization.

Several options for channel size, tissue chamber size, scaffolding and barrier design are available (see the Specifications). We can support you in your choice and select the right parameters by following your needs. 

This smart and innovative design overcomes the current limitations inherent in flow chambers or Transwell chamber based assays. Current flow chamber designs are oversimplified, lack the scale and geometry of the microenvironment and cannot model transmigration. Similarly, Transwell chambers do not account for fluid shear and size/topology observed in vivo, the end point measurements of migration are not reproducible and do not provide real-time visualization.

This simplified and idealized microvascular network easily reproduce the cellular stratification, constant shear and flow conditions.

Benefits:

• Side by side architecture enables quantitative real time visualization
• Physiological leaky vasculature with engineered porous structures
• Physiologically realistic convective and diffusive transport
• Microfluidic platform with ultra-low consumable volumes
• Highly-resistant and optically clear material
• Standard microscope glass slide size

Options (see available designs and options details in the "Specifications" tab):

• Design 1
  • Option A
    • 1) 100µm slit spacing - 2µm wide slit - 50µm barrier width
    • 2) 50µm slit spacing - 3µm wide slit - 50µm barrier width
    • 3) 50µm slit spacing - 3µm wide slit - 100µm barrier width
    • 4) 50µm slit spacing - 2µm wide slit - 50µm barrier width
    • 5) 50µm slit spacing - 2µm wide slit - 100µm barrier width
  • Option B
    • 1) 3µm pillar diameter - 3µm gap - 100µm barrier width
    • 2) 3µm pillar diameter - 8µm gap - 50µm barrier width
    • 3) 3µm pillar diameter - 3µm gap - 50µm barrier width
    • 4) 3µm pillar diameter - 8µm gap - 100µm barrier width
    • 5) 8µm pillar diameter - 3µm gap - 100µm barrier width
    • 6) 8µm pillar diameter - 8µm gap - 50µm barrier width
• Design 2
  • Option A
    • 1) 50µm slit spacing - 2µm wide slit - 100µm barrier width
  • Option B
    • 1) 3µm pillar gap
    • 2) 8µm pillar gap