Organ-on-a-Chip片上器官完整設置

說明

一個理想的芯片上的器官應該易于使用，并且可以可逆地訪問芯片中的細胞進行下游分析。因此，我們的芯片器官滿足所有這些要求有小包裝（1個芯片-1個夾子-4個補丁）或大包裝（4個芯片-1個夾子-16個補丁）。

該微流控平臺由三部分組成：

微流控芯片

夾緊

文化補丁

模塊化微流控芯片由兩塊PC（聚碳酸酯）塑料板組成，每一塊都有四塊可逆組裝用集成磁鐵和一個彈性PDMS薄膜，以確保在8千帕壓力下良好密封。芯片上部的luer連接器允許藥物篩選和/或細胞分化實驗期間的介質灌注和動態流動條件。

包括在組件中的卡箍確保了高流量壓力（高達100千帕）下的良好密封。該芯片可以放置在一個手動螺紋夾鉗中進行組裝和快速操作，并且在動態流動條件下的實驗結束時，可以通過拆卸夾鉗和微流控芯片輕松地移除組織補片。

培養貼片是納米纖維的單層，支持功能性三維微組織中細胞的組織培養貼片包括在設置中，納米纖維可以由兩種不同的天然聚合物制成，以模擬細胞外基質：

明膠

膠原蛋白

請聯系我們，如果您想要一個由合成生物相容性聚合物制成的定制培養貼片：

聚乳酸-乙醇酸共聚物

聚己內酯

聚二甲基戊二酰亞胺

因此，可以使用不同的貼片在培養皿或微孔板中培養細胞或分化人類誘導多能干細胞（hipsc），以創建組織貼片（如心臟、神經元等）。然后，獲得的組織貼片（或其他組織培養插入物）可以集成到芯片上組裝的器官中。兩種不同細胞類型的共培養也是可能的為了在細胞兩側播種，需要一層基質凝膠和膠原蛋白混合比例取決于電池類型。

如果您想了解有關可用區域性修補程序的更多信息，請單擊此處！

安裝程序

組裝完成后，微腔和微通道在芯片的兩個塑料板中形成圖案，以創建兩個獨立的微流控系統：由組織貼片分離的上腔和下腔。這兩個微流控室可以用不同的培養基進行灌注。這樣就可以形成一個組織界面來模擬肺泡、胃、腸、腎、肝、腦血、皮膚功能等。芯片內的組織可以通過顯微鏡觀察或采集用于下游分析。

Organ-on-a-Chip片上器官完整設置

Description

An ideal Organ-on-a-Chip should be easy to use and with a reversible access to cells in chip for downstream analysis. Hence our Organ-on-Chip meets all these demands. Available in Small pack (1 chip - 1 clamp - 4 patches) or Large pack (4 chips - 1 clamp - 16 patches).

Three components characterize this microfluidic platform:

• Microfluidic chip
• Clamp
• Culture patch

The modular microfluidic chip consists in two plastic plates in PC (Polycarbonate), each of these has four integrated magnets for reversible assembly and an elastomeric PDMS thin film to ensure good sealing up to pressures of 8 kPa. The Luer Connectors in the upper part of the chip allow medium perfusion and dynamic flow conditions during your experiments of drug screening and/or cell differentiation.

The clamp, included in the pack, ensures good sealing with high flow pressures (up to 100 kPa). The chip can be placed in a hand screwed clamp for assembling and fast operation and, at the end of your experiment in dynamic flow conditions, the tissue patch can be easily removed by disassembling the clamps and the microfluidic chip. 

The culture patch is a monolayer of nanofibers and support the organization of cells in a functional 3D microtissue. Culture patches are included in the setup and nanofibers can be made of two different natural polymers to mimic the extracellular matrix:

• gelatin
• collagen

Please, contact us if you want a custom culture patch made of synthetic biocompatible polymers:

• PLGA poly(lactic-co-glycolic acid)
• PCL polycaprolactone
• PMGI polydimethylglutarimide

Thus, different patches can be used to culture cells or to differentiate Human Induced Pluripotent Stem Cells (hiPSC) in dish or microplates in order to create your tissue patch (e.g. cardiac, neuron, etc). Then, the obtained tissue patch (or other tissue culture inserts) can be integrated in the assembled Organ-on-Chip. A co-culture of two different cell types is possible as well. In order to seed the cells on both sides a coating of matrigel and collagen is needed. The ratio of the mix will be cell type dependent. 

Click here if you want to know more about the available culture patches!

Setup

Once the assembly is completed, micro-chambers and micro-channels are patterned in both plastic plates of the chip to create two independent microfluidic systems: the upper and the lower chambers separated by the tissue patch. These two microfluidic chambers can be perfused with different culture media. Thus a tissue interface can be formed to simulate alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be observed by microscopy or harvested for downstream analysis.