Culture Patch片上器官培養貼片

文化補丁

培養貼片包括沉積在支撐微框架上的納米纖維單層。該培養貼片可用于三維細胞培養和組織形成。它允許細胞在復雜的3D組織中的自組裝，使外源性接觸小化，并大化細胞攝取。

納米纖維可以由不同的天然和合成材料制成：

明膠，

膠原蛋白，

聚乳酸-乙醇酸，

聚己內酯，

聚二甲基戊二酰亞胺

納米纖維的取向可以是：

隨機的

對齊的

支撐納米纖維的蜂窩微框架可以制成不同的尺寸、形狀和彈性您可以在兩種建筑材料之間進行選擇：

聚乙二醇二丙烯酸酯（1.2兆帕的年輕模塊）

聚二甲基硅氧烷

為了培養你自己的三維細胞培養，貼片可以簡單地放在培養皿、多孔、博伊登室中，或者直接放在兼容的微流控芯片中（單擊此處獲取有關芯片上器官設置的更多信息）在實驗結束時，可以從設備上移除貼片以進行進一步分析（如成像、蛋白質組學和基因組學）。

Culture Patch片上器官培養貼片

Culture Patch

A culture patch consists in a monolayer of nanofibers deposited on a supporting micro-frame. This culture patch can be used for 3D cell culture and tissue formation. It allows the self-assembly of cells in complex 3D tissues minimizing the exogenous contact and maximizing the cellular uptake.

Nanofibers can be made of different natural and synthetic materials:

• gelatin,
• collagen,
• PLGA poly(lactic-co-glycolic acid), 
• PCL polycaprolactone,
• PMGI polydimethylglutarimide

The orientation of nanofibers can be:

• Random
• Aligned

Honeycomb micro-frames that support the nanofibers can be made with different size, shape and elasticity. You can choose between two building materials:

• PEGDA poly(ethylene glycol)diacrylate (Young module of 1.2 MPa)
• PDMS polydimethylsiloxane (Young module of 0.5 MPa)

To grow your own 3D cell culture, the patch can be simply placed in a Petri-dish, multi-wells, Boyden chambers or directly in a compatible microfluidic chip (click here for more information on the Organ-on-Chip setup). At the end of your experiment, the patch can be removed from the device for further analyses (e.g. imaging, proteomics and genomics).